Bioanalytical method development, validation and quantification of flutamide in spiked rat plasma by using high-performance liquid chromatography

Abstract Background A quick, low cost and precise method was developed validated for flutamide in the rat plasma using a high-performance liquid chromatography. Acetanilide used as internal standard study. Flutamide extracted from methanol. The chromatographic analysis carried out on Cromasil C18 column at 227 nm methanol–water mixture mobile phase flow rate of 0.8 ml/min. Results demonstrated good linearity over 100–1000 ng/ml concentration range with regression coefficient 0.9947. accuracy found to be 97–101%, while its precision less than 5%. Limits detection quantitation were determined 2.52 7.66 ng/ml, respectively. showed recovery about 96–100% further investigate pharmacokinetics marketed formulation. Conclusions From results, it can concluded that has potential successfully determine establish pharmacokinetic parameters other flutamide-containing formulations.